Yokohama National University, Faculty of Engineering
(Bio Microsystem lab)

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● Tissue engineering

Surface research


Surface design for selective cell catch-and-release using electrochemical reaction

 Sorting of specific cells from a heterogeneous suspension is indispensable in various applications such as regenerative medicine and cancer research. Dynamic control of the biointerface between adherent cells and materials may provide a promising approach for the detachment and manipulation of cells in vitro. In this study, we developed the surface modified with zwitterionic oligopeptides and aptamers, with which cells can be selectively attached and then rapidly detached by the application of an electrochemical potential (Figure 1).


 We designed a zwitterionic oligopeptide, CGGGKEKEKEK, for preparation of cell repulsive surface. This peptide spontaneously bonded to a gold surface via a gold-thiolate bond and formed a dense molecular monolayer by the electrostatic force between neighboring molecules due to the alternating charged lysine (K) and glutamic acid (E), making the surface cell repulsive. To selectively catch cells, the surface of peptide layer was further modified with an aptamer which has high affinity for Hep G2 cells. Hep G2 cells selectively attracted on the surface were subsequently detached by electrochemically cleaving the gold-thiolate bond and releasing the peptide layer from the gold surface. The gold surface modified with designed zwitterionic oligopeptides and aptamers can be used for selective cell sorting.

J. Enomoto et al., Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM, Scientific Reports, 7, pp43375 (2017)




● Vascular
● Liver
● Hair
● Pacnreas
● Bone
● Lab Chip/ MEMS
● Surface modification
● Microbe

Fukuda Lab, Faculty of Engineering, Yokohama National University