Yokohama National University, Faculty of Engineering
(Bio Microsystem lab)

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● Tissue engineering


Lab on a chip research

 

Microdevice for cell migration assays
Objective

 Cell migration is essential for both physiological and pathological processes, including would healing, inflammation, and cancer invasion and metastasis. The high-throughput screening of the genes responsible for cell migration is therefore desired for the elucidation of the mechanisms underlying their processes.

Results

 In this study, we fabricated a microdevice for cell migration assays using reverse-transfection. Reverse-transefection is a microarray?driven gene infection system and can be used to investigate roles of many genes on a single slide. However, a trade off relationship between density and distance has been a practical issue. To solve this problem, we fabricated a microdevice that confines individual reverse-transfection spots in nano-liter scale chambers and avoid cross-contamination between the spots. Further, we examined cell migration speed with this device and demonstrated that this device can be used to find out genes responsible for cell migration.


Fig. 1 Microdevice for cell migration assay using reverse transfection.
[Reference]
Junko Enomoto, Rika Takagi, Reiko Nagasaki, Hiroaki Suzuki, Satoshi Fujita, Junji Fukuda, Reverse transfection in microchamber arrays for cell migration assays, Sensors & Actuators: B. Chemical, 2014, 190, 896-899.

 

 

 

● Vascular
● Liver
● Hair
● Pacnreas
● Bone
● Lab Chip/ MEMS
● Surface modification
● Microbe
 
 
 
 

 
Fukuda Lab, Faculty of Engineering, Yokohama National University